eclipse 90i epifluorescent microscope Search Results


imr90 cell lines  (ATCC)
99
ATCC imr90 cell lines
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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90i epifluorescence microscope  (Nikon)
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Nikon 90i epifluorescence microscope
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
90i Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eclipse 90i epifluorescence microscope  (Nikon)
96
Nikon eclipse 90i epifluorescence microscope
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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ds 5mc digital imaging system  (Nikon)
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Nikon ds 5mc digital imaging system
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
Ds 5mc Digital Imaging System, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence microscope  (Nikon)
96
Nikon epifluorescence microscope
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tie inverted microscope  (Nikon)
99
Nikon tie inverted microscope
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
Tie Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spectra x led light source  (Lumencor Inc)
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Lumencor Inc spectra x led light source
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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webcam logitech c905  (Logitech Inc)
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Logitech Inc webcam logitech c905
A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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dapi mounting medium  (Fisher Scientific)
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A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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photometrics coolsnap hq2 ccd camera  (Roper Scientific Inc)
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A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in <t>IMR90</t> and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.
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Image Search Results


A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in IMR90 and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.

Journal: Cellular signalling

Article Title: Alteration of Golgi Structure in Senescent Cells and its Regulation by a G protein ? subunit

doi: 10.1016/j.cellsig.2011.01.001

Figure Lengend Snippet: A. Dispersal of Golgi in stress induced senescence in HeLa cells. For trans Golgi labeling, HeLa cells were transfected with GalT- DsRed using lipofectamine 2000 (Invitrogen, Carlsbad, CA). The localization of GalT in live cells was monitored by epifluorescence microscopy. For trans Golgi network labeling, HeLa cells were grown on coverslips, treated for 24 hrs with BrdU to induce senescence, fixed and stained using the anti-TGN46 antibody (Sigma, St.Louis, MO). Top panels, basal cells, bottom panel, senescent cells (n>3). B. Replicative senescence in IMR90 and WI-38 cells. Early (16-17 PDL) and late PDL (46 PDL) IMR90 and WI38 cells were fixed and stained for TGN46 distribution. Images were acquired using widefield microscopy. Top panels, early PDL cells, and bottom panels, late PDL cells. C. Bar graph depicting the number of cells with compact or dispersed TGN in late and early PDL IMR90 and WI38 cells. Average plot of two experiments. The percentage variability across the experiments was <10%.

Article Snippet: HeLa cell line was from ATCC; WI-38 and IMR90 cell lines were from NIA Aging Cell Repository at Coriell Institute for Medical Research (Camden, NJ).

Techniques: Labeling, Transfection, Epifluorescence Microscopy, Staining, Microscopy

A. Control and BrdU treated senescent HeLa cells. B. Early and late PDLs of WI38 and IMR90 cells. In A & B cis Golgi structure was observed using anti-GM130 antibodies. Representative cells with compact or dispersed cis Golgi (n=2). C. Redistribution of α mannosidase, medial Golgi marker, in senescent HeLa cells. HeLa cells were transfected with CFP - α mannosidase. The distribution of α mannosidase was monitored by fluorescence microscopy (n=2).

Journal: Cellular signalling

Article Title: Alteration of Golgi Structure in Senescent Cells and its Regulation by a G protein ? subunit

doi: 10.1016/j.cellsig.2011.01.001

Figure Lengend Snippet: A. Control and BrdU treated senescent HeLa cells. B. Early and late PDLs of WI38 and IMR90 cells. In A & B cis Golgi structure was observed using anti-GM130 antibodies. Representative cells with compact or dispersed cis Golgi (n=2). C. Redistribution of α mannosidase, medial Golgi marker, in senescent HeLa cells. HeLa cells were transfected with CFP - α mannosidase. The distribution of α mannosidase was monitored by fluorescence microscopy (n=2).

Article Snippet: HeLa cell line was from ATCC; WI-38 and IMR90 cell lines were from NIA Aging Cell Repository at Coriell Institute for Medical Research (Camden, NJ).

Techniques: Control, Marker, Transfection, Fluorescence, Microscopy